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Prof. Gamal Abdel - Raheem Mohamed Sosa :: Publications:

Effect of Cadmium Chloride and/or Vitamin" C" on Metallothionein Gene Expression
Authors: O.A Khalifa, N. F. Alhusseini, G. A.M. Sosa, S. A Hemeda
Year: 2012
Keywords: Not Available
Journal: Journal of American Science
Volume: 8
Issue: Not Available
Pages: 198-203
Publisher: Not Available
Local/International: International
Paper Link:
Full paper Gamal Abdel - Raheem Mohamed Sosa_Effect of Cadmium Chloride.pdf
Supplementary materials Not Available

Heavy metals including cadmium represent environmental hazards and a great problem as these heavy metals lead to a great public risks for human, animal and poultry health. Metallothionein (MT) is a low molecular weight metal binding protein; it plays an important role in providing protection from cadmium toxicity. Vitamin C is one of the most important antioxidants that is used for prophylaxis and treatment of cadmium toxicity. The current study included 24 Albino rats and divided into four groups, each one includes six rats. First group was kept as controls (CON). The second one was given only vitamin C (VIC). The third was given only cadmium chloride Cdcl2 (CAD). The last group was given both vitamin C and Cdcl2 (VIC+ CAD). Metallothionein 1 and 2 mRNA expressions were measured in liver and kidney tissues by SYBR green relative quantitative Real time PCR in all samples. The Cdcl2 increases the expression of both MT1 and MT2 mRNA in liver by 4.421 and 3.827 fold and in kidney tissues by 1.095 and 1.203 respectively. The study also demonstrates that vitamin C decreases the expression of MT1 and MT2 in kidney tissues by 0.214 and 0.191 fold respectively and has no significant effect on liver tissues. It was suggested that MT and MT2 mRNA act as biomarkers of cadmium toxicity. Vitamin C had a protective role against cadmium through its antioxidant effect and that appeared through decreasing the expression of both MT1 and MT2 in liver and kidney tissues.

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