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Prof. Gamal Abdel - Raheem Mohamed Sosa :: Publications:

Evaluation of in vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques
Authors: A.E. Abd el-ghafar and G. Campanile A.R. Elkhawagah, V. Longobardi, B. Gasparrini, G.A. Sosa, G. Bifulco, M.E.A. Abouelroos
Year: 2013
Keywords: Not Available
Journal: Journal of Reproduction and Infertility
Volume: 4
Issue: Not Available
Pages: 19-28
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Gamal Abdel - Raheem Mohamed Sosa_evaluationofinvitrocapacitationofbuffalofrozenspermbydifferenttechniques_2.pdf
Supplementary materials Not Available

This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 µg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration, cleavage rates and kinetics of embryo development after heterologous IVF. The percentage of LPC-induced acrosome reacted (AR)-sperm increased (P< 0.05) with heparin compared to the control after 2 hrs (28.2 vs 24.4%, respectively) and 4 hrs (35.1 vs 32.0%, respectively). No differences in CTC pattern B (capacitated sperm) were found between groups and incubation times (on average 63%). On the contrary, heparin decreased (P< 0.01) the percentage of tyrosine phosphorylation pattern A after 2 and 4 hrs (34.3 and 35.3%, respectively) compared to the control (54.5 and 51.8%, respectively) and increased (P< 0.01) that of pattern EA after 2 and 4 hrs (59.2 and 54.2%, respectively) compared to the control group (44.7 and 45.2%, respectively). Both cleavage and penetration rates, as well as the percentage of fast developing embryos, were higher (P< 0.01) in the heparin-treated group (77.2, 80.4 and 74.0%, respectively) compared to the control (56.6, 58.0 and 55.2%, respectively).

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