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Prof. Gamal Abdel - Raheem Mohamed Sosa :: Publications:

Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection
Authors: Al-Shimaa A.El-Naby ; Mahmoud, Karima Gh M; El-Raey, M; Ahmed, YF; Abouel-Roos, MEA; Sosa, Gamal AM
Year: 2017
Keywords: Not Available
Journal: Egypt. J. Vet. Sci.
Volume: 48
Issue: Not Available
Pages: 43-51
Publisher: Not Available
Local/International: Local
Paper Link: Not Available
Full paper Gamal Abdel - Raheem Mohamed Sosa_ImpactofUsingBrilliantCresylBlueStainonOocyteandEmbryoSelection_3.pdf
Supplementary materials Not Available

T HE SUCCESS of in vitro embryo production is ultimately dependent on the number and quality of cumulus-oocyte complexes harvested from ovaries. The morphological criteria are routinely used for oocytes selection in most laboratories. There is brilliant cresyl blue stain (BCB) test allowing the selection of developmentally competent oocytes which is a simple, quick, economic and feasible protocol. BCB is known to be a non-invasive methodology that allows the selection of immature oocytes in several species. BCB test determine the intra cellular activity of glucose-6-phosphate dehydrogenase (G6PDH) that gradually decreases its activity as oocytes reach their growth phase. The using of BCB test before IVM is still controversial, so this review describes the use of BCB staining test for oocytes and embryo selection to investigate the impact of application of this stain in in-vitro maturation and embryo production in animals.

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