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Prof. Gamal Abdel - Raheem Mohamed Sosa :: Publications:

Influence of Epidermal Growth Factor with Cysteamine on in-Vitro Buffalo Embryo Development
Authors: Al-Shimaa A.H. El-Naby, Karima Gh. M. Mahmoud, T.H. Scholkamy, Y.F. Ahmed, G.A.M. Sosa and M.E.A. Abouel-Roos
Year: 2016
Keywords: Not Available
Journal: Egypt. J. Vet. Sci.
Volume: 47
Issue: Not Available
Pages: .27-39.
Publisher: Not Available
Local/International: Local
Paper Link: Not Available
Full paper Gamal Abdel - Raheem Mohamed Sosa_Influence of Epidermal Growth Factor with.pdf
Supplementary materials Not Available

FOR IMPROVING embryo development in buffalo, two experiments were conducted. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. The selected oocytes were cultured in the four concentrations of EGF. The embryo cleavage rate was significantly higher in oocytes exposed to 5, 25, 50 ng/ml EGF than control. There were no significant difference in four groups (0, 5, 25, 50 ng/ml EGF) in the rate of morula. But, better cleavage and blastocyst rates were observed at 5 ng/ml EGF. In the second experiment, the additive effect of 5 ng/ml EGF with 50 μM cysteamine on maturation and embryo development was studied. Oocytes were collected, matured and cultured in three groups. In the first group, media supplemented with 5 ng/ml EGF + 50 μM cysteamine combination. In the second group, media supplemented with EGF. The third group was supplemented with cysteamine. There was a significant increase in cleavage rate in combination group than EGF (P< 0.05) and cysteamine (P< 0.01) groups. But there was no significant difference in cleavage rate between EGF and cysteamine. The morula percentage was nearly similar in the three groups. But blastocyst rate was significantly (P

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