The current study was designed to find out the effect of different doses (8, 12 or 16 µg) of GnRH analogue (Buserelin acetate, BA) on the ultrasonographic picture and biometry of buffalo bulls’ reproductive organs. Animals (n= 9, aged 15-18 months) were allocated into three groups (n= 3/group) according to the hormonal dose that was administered once weekly for six weeks. Results revealed that the testicular diameter and epididymal tail width did not differ significantly before and after treatment. The epididymal tail length did not differ significantly before and after treatment with the 8µg, while it differed significantly (P< 0.05) with doses of 12 and the 16 µg GnRH. The ampulla ductus deference and seminal vesicle heights differed significantly (P< 0.05) before and after treatments. The prostatic body differed significantly (P< 0.05) after treatments with 8 and 16 µg. The pars disseminate of prostate gland and the bulbourethral gland height did not differ significantly regardless the dose of treatment. The bulbourethral gland length differed significantly after treatment with the 8 µg, while it did not show significant differences with doses of 12 and the 16 µg of GnRH. In conclusion, the present results demonstrated that GnRH injection at a dose of 12 µg efficiently affect buffalo bulls reproductive organs. Moreover, ultrasonography is an affirmative indicator to the response of male reproductive system to exogenous GnRH analogue treatment. |
