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Prof. Mohamed Hassan Refaat Ismail :: Publications:

ESTs analysis of the sugar beet (Beta vulgaris L.) responsive transcripts under salt stress.
Authors: Faheem M. M. ;Abd El-Maksoud R. M. ; Abd-Elgwad B. A.; Refaat M.H ;El-Akkad T.A.
Year: 2018
Keywords: Salinity stress, Differential Display Reverse Transcriptase (DDRT-PCR), EST, Gene expression, Beta vulgaris
Journal: Arab J. Biotechnology
Volume: 21
Issue: 2
Pages: 115-126
Local/International: Local
Paper Link:
Full paper Mohamed Hassan Refaat Mohamed Ismail_115-126-2018.pdf
Supplementary materials Not Available

Differential Display Reverse Transcriptase (DDRT-PCR) technique was used to analyze differentially expressed genes in sugar beet (Beta vulgaris L.) under salt stress. Three weeks old seedlings were exposed to salt stress with 100mM and 300mM NaCl, and untreated seedlings were used as control. Thirty-three differentially expressed fragments were identified and characterized. The fragments were classified according to their time of expression after the drought stress. The significance of the function of the identified differentially expressed genes was discussed in relation to their possible roles as stress genes. Seven fragments showed no significant homology with any database sequences in the GenBank. Results of the database sequence alignment identified four fragments (Bv-1=506bp, Bv19=521bp, Bv26=899bp, and Bv-31=550bp) revealing significant homology with Expressed Sequence Tags(ESTs) from salt stressed sugar beet; twenty-one fragments showed significant sequence homology with drought and cold stress- responsive genes, as well as acetyl-CoA carboxylase and glycosyltransferases. These results implicate that several pathways are involved in the plant's response to drought stress which still needs to be elucidated further.

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