Phytochemical studies in relation to development and growth of viral infected N. tabaccum cv.
White burley are rather restricted. This study analyzed Tobacco mosaic tobamovirus affecting plant growth
and phytochemical compositions. Quantitative data on branch, leaves formation, stem growth and fresh
and dry weights were systematically collected at three months. It was found that the branching rate of
TMV infected plant was decline than healthy ones. On the other hand, the plant height, number and area
of leaves, fresh weight and dry matter were decreased in TMV infected plants compared to the increase
in healthy ones. The TMV infection led to slightly change quantitative and qualitative total chlorophyll,
phenolic antioxidant compounds and soluble protein in infected plant. The TMV infection leads to a
decrease in chlorophyll a + b, total phenols and soluble protein by rate 47.89, 7.89 and 61.35% in infected
leaves. On the contrary TMV infection leads to an increase in carotenes by rate 39.28% in infected
tobacco leaves. As well as increase in the phenolic antioxidant compounds in infected leaves. Twenty two
phenolic compounds were identified in the leaves of N. tabaccum cv. While burley and nineteen in healthy
ones by HPLC. N-Hydroxy acyl nornicotine, newly discovered was found in fresh shoot plants with higher
amount in healthy compared with TMV infected plants, but not in roots tissues. Nicotine was found in
fresh tissues with low concentration in infected root, stem and leaves but was found with high
concentration in healthy ones. Nornicotine was found in fresh healthy tissues of roots, stem and leaves
but no identified in TMV infected stem and leaves by TLC. |
