: Cancer is a huge global disease burden. Every year, tens of millions of people worldwide
are diagnosed with cancer, and more than half of them die as a result of it. The great biodiversity
of the marine environment has increasingly piqued the interest of experts, especially in the field of
drug discovery. The marine fungus Aspergillus fumigatus WA7S6 has been selected among a group of
fungi isolated from marine sponges as it exhibits a pronounced antimicrobial activity toward a group
of pathogenic microbes. The fungus has been identified genetically by amplification and analysis
of its 18srRNA gene. The fungus crude extract has been obtained by cultivation of the fungus on
rice media. The crude extract was tested for antibacterial activity against a variety of pathogenic
microorganisms. The results demonstrated a pronounced antimicrobial action against P. aeruginosa,
S. aureus, A. niger, and Candida albicans. Furthermore, we tested the antioxidant potential of the
Aspergillus fumigatus WA7S6 crude extract using three different methods: ATBS, DPPH, and lipid
peroxidation assays. Results showed that the crude extract WA7S6 had an IC50 value of 21.35 µg/mL.
The anticancer potential of the crude extract was also evaluated against cancer cell lines such as Hela,
MCF, and WI-38. The chemical profiling of the fungus extract was identified via GC-mass and in silico
molecular docking of the identified compounds on heme oxygenase, as a stress protein included in
cellular protection, antioxidant, and anti-inflammatory activities, suggesting that some compounds,
such as 9-Tetradecynoic acid, 11-Hexadecynoic acid, methyl ester, and dehydromevalonic lactone,
could be relevant for antioxidant purposes.
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