Egyptian garlic cultivars Sids-40 and three genotypes i.e., Chines, Italian and Brazilian were evaluated for their
productivity in two field experiments to determine the genetic divers during winter seasons of 2012/2013 and 2013/2014.
The results showed that the uppermost plant height was 100.0 and 96.3 cm for Egyptian cultivar (Balady) in both seasons,
respectively. The maximum value of leaves number were detected with Sids-40 while the largest leaf area per plant were recorded to Chinese genotype. Moreover, Chinese genotype was also greater than other cultivars and genotypes in total
plant and leaves fresh and dry weight. However, the highest chlorophyll content was obtained by Brazilian genotype.
Whereas, the highest cloves number per bulb were produced by Egyptian and Brazilian genotype, respectively. The highest
bulb diameter, average bulb weight and average clove weight were achieved by Chines genotype compared with other
cultivars and genotypes tested. The greatest TSS and allicin contents in cloves and bulbs of garlic were found in cv. Egyptian(Balady) followed by Brazilian genotype in case of allicin content. Whereas, the marketable yield weight was observed by Chines and Sids-40.
Inter-simple sequence repeat (ISSR) was assayed to determine the genetic diversity of 5 garlic genotypes. Six ISSR primers were used in current investigation. A high level of polymorphism among garlic genotypes was found with ISSR marker number 6 that also showed amplicons with all garlic genotypes under study. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies Also, the present results along with those of other researchers show that ISSRs can be used for cultivar differentiation in garlic. |
