This study aimed at displaying the histo-morphological changes in the vaginal cells and ovaries in
response to GnRH, calcium, or bromocriptine. A total of 40 lactating NZW does were assigned into four
comparable groups three of which were administered by GnRH, calcium and bromocriptine, while the fourth was
given saline as control. Changes occurring in the vaginal cells and ovaries were demonstrated. Linear
regression analysis revealed that the coefficient of determination (R2) between parabasal cells, intermediate,
superficial and anuclear cells and follicular population was 0.92, 0.42, 0.39 and 0.35, respectively. The total
ovarian follicle number was significantly lower in Br-G and GnRH-G at two and four hours post-treatment,
respectively, when compared to that in control. Ovarian interstitial tissue showed hypertrophy and hyperplasia
in the GnRH-G associated with higher rate of atresia and pyknosis in Cal-G and Br-G. From these results, it can
be concluded that exfoliated parabasal cells in the vaginal smear might be helpful to express the ovarian
function in does; prolactin suppression up to eight hours resumes the subsequent follicular growth and hence
improve fertility. Calcium administration might have a synergistic action with pituitary gonadotropins at the
ovarian level. Moreover, calcium and bromocriptine administration have the ability to increase the follicular
population in does, even though different mode of action. |
