This study aimed to estimate the effects of restriction fragment length polymorphism [RFLP] in the follicle stimulating hormone receptor gene [FSHR gene], regarding to fertile and infertile Egyptian buffaloes. The ovarian status of animals was classified by ultrasonography into follicular phase, luteal phase, bilateral inactive ovary with normal uterus and bilateral inactive ovary with endometritis. Blood samples were collected from fertile and non-fertile buffaloes with the history of anestrum or repeat breeders for DNA extraction and progesterone analysis. The results showed that, the overall mean of plasma progesterone levels in normal cyclic animals were significantly higher during the luteal phase (5.78±0.69 ng/ml) as compared to the follicular phase (1.57±0.18 ng/ml). Moreover, there were no significant differences between animals had normal uterus and those suffered from endometritis in association with bilateral inactive ovaries. All buffaloes investigated in this study were genotyped as CC where DNA amplified fragments at 306-bp were digested with AluI endonuclease and gave two digested fragments at 243- and 63-bp. In conclusion, monomorphic pattern of follicle-stimulatinghormone receptor gene [FSHR gene] is considered a unique feature that may be related to the characteristic species in buffalo. So, the polymorphisms and interaction with the fertility feature should be endorsed for advanced research with a big number of buffaloes. |
