A total of 100 random samples of meat products including raw minced meat, raw sausage, luncheon and
basterma (25 samples of each) were collected from different markets in Cairo and Giza governorates to
be examined bacteriologically for detection of Staphylococcus aureus, Listeria monocytogenes and
Escherichia coli. These samples were examined for isolation of such pathogens by conventional
bacteriological methods and by polymerase chain reaction (PCR). Concerning S.aureus bacteriological
results revealed the prevalence in minced meat, Sausage, luncheon and basterma was (24%, 24%, 20%,
4%) respectively. While L. monocytogenes revealed the prevalence in minced meat, sausage, luncheon
and basterma was (4%, 0%, 0%, 0%) respectively and E. coli revealed the prevalence in minced meat,
sausage, luncheon and basterma was (20%, 20%, 24%, 20%) respectively. The results cleared that PCR
is an ideal method for identification of foodborne pathogens, as it was effective, less labor, more
sensitive, reduces effort and time after using gradient PCR in validation of each microbe. |
