Dr. Sherif Ibrahim Ahmed Ramadan :: Publications:

A total of 229 Arabian horse hair samples including 120 samples from El-Zahraa stud and 89 from two private farms in addition to 20 hair samples of Dutch Warmblood were genotyped by 16 microsatellite markers. The purposes of this study were; firstly, to investigate the current status of the genetic diversity and inbreeding of Arabian horse populations reared in Egypt. Secondly, to examine the traditional maternal based strain classification system “Al Khamsa” using samples of native Arabian horses reared in the El-Zahraa stud based on 16 microsatellite markers. El-Zahraa stud showed high inbreeding (FIS = 0.110) and should be corrected by modifying mating system through avoiding excessive use of certain sires in breeding program. Across the five basic strains of the Arabian horse, nine loci showed 13 private alleles with the Seglawi recorded six and the Abeyan recorded no private alleles. The highest Nei genetic distance and pairwise FST values were recorded between Abeyan and Hamdani while the lowest were recorded between Kehilan and Seglawi. The cluster pattern of the individual phylogenetic tree and STRUCTURE plots of the five basic horse strains indicate that there was no sharp demarcation between those five strains, and the influence of the dame line and the traditional maternal lines classification of the El-Zahraa Arabian horses was unclear. The results of this study confirm the applicability and efficiency of these 16 STR markers for assessing genetic diversity but not in examining the traditional maternal based strain classification system using native Arabian horses from Egypt.