You are in:Home/Publications/Prenatal exposure to DEHP induces premature reproductive senescence in male mice

Prof. Mohamed El Sayed Sobhey Abosalem :: Publications:

Prenatal exposure to DEHP induces premature reproductive senescence in male mice
Authors: Radwa Barakat1,3, Po-Ching Patrick Lin1, Saniya Rattan1, Emily Brehm1, Igor F. Canisso2,Mohamed E. Abosalem3, Jodi A. Flaws1, Rex Hess1 and CheMyong Ko1,*
Year: 2017
Keywords: DEHP Endocrine disruptor Fertility Testes Semen
Journal: Toxicological Sciences
Volume: 10.
Issue: 1093/toxsci/kfw
Pages: 248
Publisher: Oxford University Press on behalf of the Society of Toxicology.
Local/International: International
Paper Link:
Full paper Not Available
Supplementary materials Not Available

Di-(2-ethylhexyl) phthalate (DEHP) is the most commonly used phthalate, and it is an endocrine-disrupting chemical. This study tested a hypothesis that prenatal exposure to DEHP lays the foundation for premature gonadal dysfunction and subsequent reproductive senescence in male mice. Pregnant female CD-1 mice were orally dosed with vehicle control (tocopherol-stripped corn oil) or with 20 μg/kg/day, 200 μg/kg/day, 500 mg/kg/day or 750 mg/kg/day of DEHP from gestational day 11 to birth. Overall, the prenatal DEHP exposure did not cause any overt physical health problems in male offspring, as no significant differences in their body nor gonadal weight were seen up to the age of 23 months. However, an age- and dose-dependent gonadal dysfunction was observed. As early as 7 months of age, the 750 mg/kg/day group of mice exhibited significantly reduced fertility. At 19 months of age, 86% of the 750 mg/kg/day mice became infertile, whereas only 25% of the control mice were infertile. At this age, all of the DEHP-exposed mice had lower serum testosterone levels, higher serum estradiol levels, and higher LH levels compared to control mice. Histological evaluations showed that mice prenatally exposed to DEHP displayed a wide array of gonadal and epididymal abnormalities such as increased germ cell apoptosis, degenerative seminiferous tubules, oligozoospermia, asthenozoospermia, and teratozoospermia in comparison to age-matching control mice. In summary, this study shows that prenatal exposure to DEHP induces premature reproductive senescence in male mice.

Google ScholarAcdemia.eduResearch GateLinkedinFacebookTwitterGoogle PlusYoutubeWordpressInstagramMendeleyZoteroEvernoteORCIDScopus