Biological Studies On Herpes Simplex Virus :

Mahamed Gouda Awadalla

Primary infections with HSV lead to theestablishment of a perminant latent infectionwhere the virus hides in the nerve ganglia. Viruscan be reactivated from the dormant stage anda recurrent infection with clinical or subclinicalmanifestations result. Both a primary and a recurrentinfection stimulates the humoral and cellularimmune systems. Antibodies specific HSV are producedearly in the infection and the immunoglobulinsof IgA, IgM and IgG classes are demonstrable.In the present work, an ELISA technique was usedto detect HSV-I and II specific immunoglobulinclasses in both serum and saliva.The study was performed on two age groups:-1-64 adults aged between 16-24 years.2-61 children aged between 6-24 months.All these subjects were apparently healthy.1]IgM First qroup and IgG inTested for HSV-I specific IgA,serum and saliva and the resultswere as follows* 68.9% were +ve for HSV-1 specific 19A in serumand saliva.* 8.2% were +ve for HSV-1 specific 19G in serumand saliva.* 1. 6% were +ve for HSV-1 specific 19M in serumand saliva.2] Second group Tested for HSV-11 specific19A, 19G and 19M in serum and saliva and theresults were as follows :* 87.5% were +ve for HSV-11 specific 19A in serumand saliva.* 31.3% were +ve for HSV-11 specific 19G in serumand saliva.* 31.3% were +ve for HSV-11 specific 19M in serumand saliva.from the results, it was found that HSVspecific19A is the most common immunoglobulindetected in both serum and saliva followed by19G then 19M.Also, the results obtained from both serumand saliva are nearly similar which reflectsthe feasability of using saliva for diagnosisof HSV-infection or for serological surveys.