Role Of Peritoneal Fluid In Unexplained And Endometriosis Associated Female Infertility:

Umnia Abd El-kader El-hendy

The present study included 90 female patients attending obstertics and gynecology departments, Benha University Hospitals, Egypt andHutzel Hospital, Detroit, Michigan, USA. They were divided into 3groups. Group I: 30 patients of mild endometriosis, Group II: 30 patientsof unexplained infertility and Group III formed of 30 control fertilefemales. For each patient a full history was taken, and a thorough generalabdominal and pelvic examination was done. All infertility factors wereevaluated for each patient in the first and second groups. Male fertilitywas evaluated by complete semen analysis. A postcoital test wasperformed to exclude cervical factor. A premenstrual endometrial biopsywas taken and examined histo-pathologically for detection of ovarianfunction. Hysterosalpingography was performed in the proliferative phasefor evaluation of uterine and tubal factor. The peritoneal fluid dampleswere collected during laparoscopy at the proliferative phase of the cycle.PF samples were subjected for estimation of the total proteinconcentration, detection of different protein bands by SDS - PAGE,qualitativen and quantitative estimation of the cytokine TNF-a bywestern blotting and ELISA, in addition to study the effect of PF on 2-cell mouse embryos development.Our results revealed that:[J Total protein concentration showed statistically significantmcreases in endometriotic group compared to the control andunexplained infertility groups (P