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Prof. Fahim Aziz Eldin Mohamed Shaltout :: Publications:

Title:
Plasmid profile analysis of Salmonellae isolated from some meat products.
Authors: Edris, A.M.; Shaltout, F.A. ;Salem, G.H. and El-Toukhy,E.I.
Year: 2011
Keywords: Luncheon, Plasmid, Salmonellae, Sausage, Minced Meat.
Journal: Benha veterinary medical journal
Volume: Not Available
Issue: Not Available
Pages: 172-178,
Publisher: faculty of veterinary medicine , benha university
Local/International: Local
Paper Link: Not Available
Full paper Fahim Aziz Eldin Mohamed Shaltout_Fahim Aziz Eldin Mohamed Shaltout_949c74de8b116b3992b1a951d635d1be8502 (1).pdf
Supplementary materials Fahim Aziz Eldin Mohamed Shaltout_Fahim Aziz Eldin Mohamed Shaltout_949c74de8b116b3992b1a951d635d1be8502 (1).pdf
Abstract:

A total of 300 random samples of luncheon, sausage and frozen minced meat (100 of each) were collected from different supermarkets at Qalyubia governorate. The samples were transferred directly to the laboratory under strict hygienic conditions and bacteriologically examined for detection and plasmid profile analysis of salmonellae. This work determined the plasmid profiles of the salmonella serovars by using ethidium bromid stained 1% agarose gel electrophoresis. In this study, plasmid analysis revealed that the isolated salmonella serotypes carry different numbers of plasmids with variable molecular weights ranged from 1233.18 to 15280.82 base pair (bp). The obtained results revealed that S. typhi, S. typhimurium and S. enteritidis contain a common molecular weight band at 7532.35 bp. and the number of plasmids ranged from 4 to 5 bands. Moreover, S. typhi and S. typhimurium sharing the same band at 1233.18 bp. In the current study, S. enteritidis and S. typhimurium were recognized to have common band at 1631.12 bp. The obtained results revealed that S. typhi, and S. enteritidis have 4 bands but S. typhimurium have 5 bands.

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