Role Of Anti-oxidant Vitamins A And C In Toxicity Prevention In Some Vital Orcans Of Albino Rat:

Mohamed Mahmoud Yousef

The present study was conducted on 80 male n .ts to probethe effect of vitamins A and C, solely and in com iination, onthe recovery of artificially-toxified liver and on b me marrowcells.Animals were divided into five groups as follows :-Group I (control group)Formed of 10 rats. Each animal was given only 0.5 ml ofdistilled water every day by an oral tube.Group II (positive control group)Formed of 10 rats. Each animal was given a weekly oral doseof CC4 after 2 weeks from the start of the experi ment for 8weeks.Group III (Vitamin A experimental group) •Formed of 20 rats and divided into two subgroups eachcontaining 10 rats:- Subgroup (a): Each animal was given daily orall dose ofvitamin A for 2 weeks then CCI4 for 8 weeks.- Subgroup (b): Each animal was given daily oral dose ofvitamin A for It.weeks while CCl4 was given for 8 weeksstarting 2 weeks after the initial dose of vitamin A.~CgnclusionGroup IV (Vitamin C experimental group) :Formed of 20 rats and divided into two subgro ’fpscontaining 10 rats:_Subgroup (a): Each animal was given daily 0vitamin C for 2 weeks then CCl4 for 8 weeks._Subgroup (b): Each animal was given daily orvitamin C for Itweeks while CC4 was given f !rstarting 2 weeks after the initial dose of vitamin C.eachdose ofdose of8 weeksGroup V (Vitamin A&C experimental grou )Formed of 20 rats and divided into two sub oups eachcontaining 10 rats:_Subgroup (a): Each animal was given daily 0 I dose ofvitamin A and C for 2 weeks then CCl4 for 8 wee_Subgroup (b): Each animal was given daily or I dose ofvitamin A and C for Itl weeks while CCl4 was given for8 weeks starting 2 weeks after the initial doses of .tamins.* Liver and bone marrow samples were taken 2,4 5,8 and 10weeks after the initial dose of CC4 (the last s mple wasconsidered as a withdrawal sample taken 2 leeks afterstoppage of CCI4).* from the liver samples, histological sections we l preparedpd Conclusionfor light and EM study and from the bone marro samples,chromosomal assay and transformed cell study were performed,The results of the present study revealed the fo lowing:1- Vitamin C had an evident role in preventi g the signsof toxicity on hepatocytes and hepatic lob les whilevitamin A had a drastic effect on such to .icity to thelevel that it inhibited the antioxidant role of vitamin Cwhen combined.2- Neither vitamin A nor vitamin C had shown increase inchromosomal abnormalities when used s I.ely or incombination and the chromosomal abnormaliti s remainedwithin normal range.3- Relative to positive control group both vitami 1S A and Csignificantly increased the percentage of the ransformedlymphocytes (P < 0.01) while combined vi in A and Cshowed highly significant increase in ransformedlymphocytes (P < 0.005).4- Pretreatment with either vitamin A or vitami C solely orcombined showed no prophylactic effect 11 the livertoxicity, chromosomal abnormalities or the p rcentage ofthe transformed lymphocytes.