A total of 300 random samples of luncheon, sausage and frozen minced meat (100 of each) were
collected from different supermarkets at Qalyubia governorate. The samples were transferred directly
to the laboratory under strict hygienic conditions and bacteriologically examined for detection and
plasmid profile analysis of salmonellae. This work determined the plasmid profiles of the salmonella
serovars by using ethidium bromid stained 1% agarose gel electrophoresis. In this study, plasmid
analysis revealed that the isolated salmonella serotypes carry different numbers of plasmids with
variable molecular weights ranged from 1233.18 to 15280.82 base pair (bp). The obtained results
revealed that S. typhi, S. typhimurium and S. enteritidis contain a common molecular weight band at
7532.35 bp. and the number of plasmids ranged from 4 to 5 bands. Moreover, S. typhi and S.
typhimurium sharing the same band at 1233.18 bp. In the current study, S. enteritidis and S.
typhimurium were recognized to have common band at 1631.12 bp. The obtained results revealed that
S. typhi, and S. enteritidis have 4 bands but S. typhimurium have 5 bands. |
