Fahim Aziz Eldin Mohamed Shaltout|Publications:Isolation and identification of Salmonella and E,coli isolated from cut

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Prof. Fahim Aziz Eldin Mohamed Shaltout :: Publications:

Title:	Isolation and identification of Salmonella and E,coli isolated from cut - up meat and chicken by using PCR
Authors:	Saad, M.S., Edris, A.M., Shaltout, F.A. and Edris-Shimaa, N.
Year:	2011
Keywords:	E. coli, Meat, Multiplex PCR, Salmonella.
Journal:	Benha veterinary medical journal
Volume:	22
Issue:	2
Pages:	152-160
Publisher:	faculty of veterinary medicine , benha university
Local/International:	International
Paper Link:	http://bvmj.bu.edu.eg/abstract.php?id=43&abstract=%20A%20total%20of%20100%20random%20samples%20of%20chicken%20(thigh%20and%20breast)%20and%20red%20meat%20cuts%20(mutton%20and%20beefshoulders)%20were%20collected%20from%20different%20poulterer%EF%BF%BDs%20and%20butcher%EF%BF%BDs%20shops%20at%20Cairo,%20El-%20Kalyobia%20andEl-Gharbia%20governorates%20to%20detect%20level%20of%20Salmonella%20and%20E.coli%20contamination.%20The%20obtained%20resultsindicated%20that%20salmonella%20organisms%20were%20isolated%20from%20the%20examined%20samples%20of%20chicken%20thigh,chicken%20breast,%20mutton%20and%20beef%20with%20percentages%20of%2016%,%2016%,%208%%20and%208%%20respectively.%20Moreover,the%20isolated%20Salmonellae%20could%20be%20serologically%20identified%20as%20S.%20Typhimurium%20(28%),%20S.%20Enteritidis(16%)%20and%20S.%20Haifa%20(4%).%20On%20the%20other%20hand,%20the%20percentages%20of%20isolated%20E.%20coli%20from%20the%20examinedsamples%20of%20chicken%20thigh,%20chicken%20breast,%20mutton%20and%20beef%20were%2016%,%2012%,%2028%and%2012%%20respectively.Moreover,%20the%20results%20cleared%20that%20PCR%20is%20an%20ideal%20method%20for%20identification%20of%20Salmonella%20spp.%20as%20itwas%20effective,%20less%20labor%20and%20more%20sensitive%20as%20well%20as%20reduces%20effort%20and%20time.%20Out%20of%2010%20strains%20ofdifferent%20serotypes%20of%20Salmonella%20isolated%20from%20chicken%20(thigh%20and%20breast),%20mutton%20and%20beef%20bytraditional%20method,%204%20strains%20were%20positive%20in%20m-PCR%20for%20Salmonella%20from%20which,%20one%20strain%20wasidentified%20as%20S.%20Typhimurium.%20As%20well%20as%20out%20of%2010%20strains%20of%20different%20serotypes%20of%20E%20.coli%20isolatedfrom%20chicken%20(thigh%20and%20breast),%20mutton%20and%20beef%20shoulders,%202%20strains%20were%20positive%20in%20m-PCR.%20E.coliO55:%20K59%20(B5)%20and%20E.coli%20O119:%20K69%20(B14)%20isolated%20from%20thigh%20and%20breast,%20respectively,%20which%20werepositive%20for%20elt%20gene%20(labile%20toxin).&keywords=E.%20coli,%20Meat,%20Multiplex%20PCR,%20Salmonella.&title=ISOLATION%20AND%20IDENTIFICATION%20OF%20SALMONELLAE%20AND%20E%20.COLIFROM%20MEAT%20AND%20POULTRY%20CUTS%20BY%20USING%20MULTIPLEX%20PCR&author_name=Saad,%20M.S.,%20Edris,%20A.M.,%20Shaltout,%20F.A.%20and%20Edris-Shimaa,%20N.&viewed=0
Full paper	Fahim Aziz El-Dein Mohamed Shaltout_17_2.pdf
Supplementary materials	Fahim Aziz El-Dein Mohamed Shaltout_17_2.pdf

Abstract:

A total of 100 random samples of chicken (thigh and breast) and red meat cuts (mutton and beefshoulders) were collected from different poulterer�s and butcher�s shops at Cairo, El- Kalyobia andEl-Gharbia governorates to detect level of Salmonella and E.coli contamination. The obtained resultsindicated that salmonella organisms were isolated from the examined samples of chicken thigh,chicken breast, mutton and beef with percentages of 16%, 16%, 8% and 8% respectively. Moreover,the isolated Salmonellae could be serologically identified as S. Typhimurium (28%), S. Enteritidis(16%) and S. Haifa (4%). On the other hand, the percentages of isolated E. coli from the examinedsamples of chicken thigh, chicken breast, mutton and beef were 16%, 12%, 28%and 12% respectively.Moreover, the results cleared that PCR is an ideal method for identification of Salmonella spp. as itwas effective, less labor and more sensitive as well as reduces effort and time. Out of 10 strains ofdifferent serotypes of Salmonella isolated from chicken (thigh and breast), mutton and beef bytraditional method, 4 strains were positive in m-PCR for Salmonella from which, one strain wasidentified as S. Typhimurium. As well as out of 10 strains of different serotypes of E .coli isolatedfrom chicken (thigh and breast), mutton and beef shoulders, 2 strains were positive in m-PCR. E.coliO55: K59 (B5) and E.coli O119: K69 (B14) isolated from thigh and breast, respectively, which werepositive for elt gene (labile toxin).

Prof. Fahim Aziz Eldin Mohamed Shaltout :: Publications: