Sixty samples of random meat products luncheon, basterma and hawawshi (20 of each) were
collected from different localities in Kalyobia governorate, Egypt. The collected samples were
subjected to mycological examination, detection of aflatoxin B1 residues as well as the ability of
isolated A.flavus for production of aflatoxin B1 and identification of toxigenic strains by PCR. The
obtained results revealed that the examined luncheon samples had the highest mould count with a
mean value 4.7x102± 2.1 x102
followed by basterma samples 3.4x102± 8.6x10 and Hawawshi
samples had lower mould count 7.9 x10 ± 5.2 x10. Six mould genera could be detected and
identified. The identified mould genera were belonging to genus Aspergillus, Penicillium,
Cladosporium, Mucor, Eupenicillium and Talaromyces. The highest incidence of isolate among
Aspergillus spp. was A. niger followed by A.flavus and A.parasiticus. The average concentration of
aflatoxin B1 (µg/kg) in luncheon, basterma and hawawshi were 1.4± 0.63, 0.8 ±0.47 and 0.7± 0.36,
respectively. Toxigenic A.flavus were subjected to PCR identification. Three A.flavus toxigenic
isolates were examined by polymerase chain reaction (PCR) with using specific primer (PEPO1
&PEPO2). PCR products of A.flavus strains were positive on agarose gel electrophoresis of PCR
amplification products showing 200 bp. The public health importance of the isolated moulds and
aflatoxins and the recommended points were discussed |
